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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial position in marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two added splice variants had been claimed but specific nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind exclusively towards the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded by the antibody.
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This guidebook outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best outcomes are...
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This tutorial outlines further probable optimization of cellular and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide not bind for the epitope over the target protein. By evaluating the staining within the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family members, whose associates are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which add to the temporal coordination of each mitotic function. Cyclin E types a complex with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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